xcelligence rtca mp system

The RTCA MP Instrument is composed of four main components: ... from clinical related pathogens xCELLigence in personalized cancer medicine Investigating the hallmarks of cancer using xCELLigence system … RTCA xCELLigence technology provides a great tool to unravel the entire course of a cytotoxic response without loosing any meaningful time points. The MP (multiple plate) model differs from our other xCELLigence instruments in that it can host up to six 96-well electronic microtiter plates (E-Plate® 96). * T he RTCA HT Plate Station integrat es with robotic platfor ms, and is not designed for placement in an incuba tor. Sales, info@ols-bio.de Because this phenomenon is dependent upon the electrodes interacting with bulk solution, the presence of adherent cells at the electrode-solution interface impedes electron flow. Less cell stress: Precise and standardized pacing conditions result in improved maturation of hiPSC cardiomyocytes in only 2-3 weeks. B) Virus-induced CPE, indicated by changes in cellular morphology, cell surface attachment, cell-cell adhesion and decreased number of living cells, lead to a decrease of electrical impedance, measured by gold biosensors in real time. Cell migration assays using the xCELLigence DP system have proven indispensable for our work, as it allows us to generate valuable, easily quantiﬁable kinetic data, combined with a simple and time-saving setup procedure. As control, T47D cells were incubated with only the E-Plate Insert, which had no impact on cell proliferation (red curve) as shown by stable Cell Index values. Environmental XPRT is a global environmental industry marketplace and information resource. If you continue using this website, we‘ll assume that you are happy to receive all cookies. RTCA provides a quantitative readout of cell number, proliferation rate, cell size/shape, and cell-substrate attachment quality. Low doses of irradiation resulted only in a slight decrease and a short delay before Cell Index values of untreated control cells (red curve) were reached again. Dr. Kathrin Ringwald. Hence, the effect of signalling proteins, released by indirectly co-cultured effector cells, can easily be monitored in real time using xCELLigence RTCA. Cell Index values reflect changes in cellular impedance depending on strength of adhesion, morphology, cell number etc. Moving beyond the generic example shown above, Figure 4 shows actual real-time impedance data acquired using E-Plates in ACEA’s xCELLigence® real-time cell analysis (RTCA) instruments. Roche Applied Science has introduced ACEA Biosciences' Xcelligence RTCA MP Instrument, which needs less than 15 seconds to analyse a 96-well E Plate. Because these physical properties are the product of thousands of different genes/proteins, RTCA can provide an extremely wide field of view on cell health and behavior. RTCA Plate Stations* can be placed inside standard cell culture incuba tors (RTCA MP Plate Station is shown), creating a temperature-, CO2-, and humidity-c ontrolle d environment througho ut an experiment. When submersed in an electrically conductive solution (such as buffer or standard tissue culture medium), the application of an electric potential across these electrodes causes electrons to exit the negative terminal, pass through bulk solution, and then deposit onto the positive terminal to complete the circuit. For the first few hours after cells have been added to a well there is a rapid increase in impedance. Migratory and invasive properties of cells can easily be analyzed using RTCA CIM (Cell Invasion & Migration) plates. Interestingly, the time course of the CPE clearly correlates with the virus titer (green: control (no viral infection); blue: 80,000 PFU VSV; red: 800,000 PFU VSV). xCELLigence® RTCA technology allows detection of minimal cellular changes, involving strength of adhesion, cell number, cell morphology and cell-cell-contacts. Each of these shortcomings is, however, overcome by the non-invasive, label-free, and real-time cellular impedance assay. This is caused by cells falling out of suspension, depositing onto the electrodes, and forming focal adhesions. I can revoke this consent at any time. As a result, a full kinetic read out of the cellular behaviour is obtained- objective and quantitative. Cells are able to influence each other – even without direct interaction. Download RTCA DP Brochure, RTCA SP (Single Plate) Transverse section of a single well of a CIM plate with adherent cells in the upper well and cells that already migrated to the lower well and attach to gold microelectrodes, covering the lower side of the microporous membrane. (C) Real-time impedance traces for NK 92 cell-mediated cytolysis of MCF7 breast cancer cells. Schematic representation of analyzing ADCC using xCELLigence Technology. In the current times, where several of us are fighting the race against the time to find the cure and prevention for COVID-19, the evaluation and the need for better technologies for virus-mediated cytopathogenicity have become more and more of relevance. While adherent target cells induce high Cell Index values, non-adherent effector cells, like natural killer (NK) cells, remain in suspension and hardly interact with the microelectrodes. When cells reached confluence, the virus was added as indicated by the black vertical line. Online product catalogs, news, articles, events, publications & more. Irradiation-induced cytotoxicity of HEK-293 cells: Data from Application Note „Irradiation-Induced Cytotoxicity“. xCELLigence System Real-Time Cell Analyzer Introduction G-coupled protein receptors (GPCRs), also known as ... by the RTCA MP Instrument. * T he RTCA … The RTCA SP (single-plate) Instrument consists of aRTCA Analyzer, a RTCA SP Station, and a RTCA Control Unit, and is designed for the use of one E-Plate 96 (a specialized 96-well plate used with the RTCA Instrument). The Agilent xCELLigence real-time cell analysis (RTCA) dual purpose (DP) system provides a unique and powerful means to monitor cells in real time … This pacing function supports the development of functionally mature cardiomyocytes. Click on RUN button and RELAX. Cytotoxicity can be caused by different inducers, like chemical substances (e.g. To investigate cell invasion processes, CIM plates can easily be coated with different matrices, like matrigel, collagen etc. Although these instructions have been â¦ These gold electrodes measure the change of impedance, which increases with the number of migrated cells attaching on the gold microelectrodes on the lower side of the membrane. User friendly software allows for real-time control and monitoring of the instrument, and includes real-time data display and analysis functions. This effect could be highly reduced by addition of hormone synthesis inhibitor Prochloraz (blue curve). Unseen or novel effects of all these cytotoxic inducers can easily be explored using xCELLigence systems. Analyze cardiomyocyte contractility, viability and electrophysiology in one run. In 2017, the ImpedanCELL platform acquired two live cell imaging systems (IncuCyte ® S3), one for each site. No. RTCA S16 drugs), immune cells (cytotoxic T-cells, natural killer cells), viruses or irradiation. XPRT. Generic real-time impedance trace for setting up and running an apoptosis assay. RTCA Plate Stations* can be placed inside standard cell culture incuba tors (RTCA MP Plate Station is shown), creating a temperature-, CO2-, and humidity-c ontrolle d environment througho ut an experiment. Long-Term High-Throughput Cytotoxicity Profiling. The cell response was recorded using the xCELLigence system or real-time cell analysis (RTCA) as a cell index (CI) and expressed as cell adherence (%) compared to control cells without treatment. Agilent ACEA xCELLigence RTCA MP W380 Real-Time Cell Analyzer ... System consists of: ACEA xCELLigence RTCA W380 Real-Time Cell Analyzer RTCA MP Multiple Plates 6-position Station (please note: only 3 plate protectors included with the listing) Dell Latitude i5 running RTCA Pro software ver 2.3 More detailed information in the Privacy Policy section, This website uses cookies and similar technologies. xCELLigence RTCA S16 (Pilot Scale) xCELLigence RTCA DP (Cell Invasion & Migration) xCELLigence RTCA SP (Single Plate) xCELLigence RTCA MP (Multiple Plates) xCELLigence RTCA HT (High Throughput Model) xCELLigence RTCA Cardio (Cardiomyocyte Contractility) xCELLigence RTCA CardioECR â¦ Download RTCA HT Brochure. In this example data, the breast cancer cell line T47D was used as target cell and indirectly co-cultured with steroid-producing adrenocortical effector cells H295R. Quantity: Select your device type: xCELLigence MPxCELLigence SPxCELLigence HTxCELLigence DPxCELLigence S16xCELLigence cardioxCELLigence cardio ECRxCELLigence iCELL, I would like to be informed in future about trends, webinars and offers from OLS by email (*) The eSight is the first system of its kind that combines high sensitivity of xCELLigence impedance-based measurement with the benefit of live cell imaging. All experi- Cells that loose their contact to the gold biosensors on the plate buttom are no longer detected as electrical insulators, resulting in a decrease of Cell Index values. The MP (multiple plate) model differs from our other xCELLigence instruments in that it can host up to six 96-well electronic â¦ By submitting my data and sending the registration, I agree to receive the newsletter ordered here by email. Figure 1. Download RTCA MP Brochure, RTCA HT (High Throughput) This high aquisition rate in the range of milliseconds enables the detection of morphological changes that are due to cardiomyocyte contractility. Unlike traditional assay endpoints, xCELLigence … Cell migration assays using the xCELLigence DP system have proven indispensable for our work, as it allows us to generate valuable, easily quantiﬁable kinetic data, combined with a simple and time-saving setup procedure. I agree that my data will be used to process my request. In general, cytotoxic responses lead to an altered cellular morphology, often accompanied with cell detachment from the well bottom. Label-Free Assay for NK Cell-Mediated Cytolysis, Real-Time, Label-Free Measurement of Natural Killer Cell Activity and Antibody-Dependent Cell-Mediated Cytotoxicity, xCELLigence RTCA-Pro Software - Software Designed for Immunotherapy Applications. The xCELLigence® RTCA MP system accommodates up to six 96-well microtiter E-Plates. (A) Simplified schematic of the interdigitated electrodes used in each well of an E-Plate. Importantly, cell behaviour is not affected by the gold microelectrodes or the electrical current which is applied. Doxorubicin, a commonly used chemotherapeutic agent, belongs to the class of drugs called anthracyclines. Cell viability was continuously analyzed every 15 minutes using xCELLigence SP system. ... (RTCA) Systems xCELLigence â¦ The MP (multiple plate) model differs from our other xCELLigence ® instruments in that it can host up to six 96-well electronic â¦ The cycle of cardiomyocyte contraction and relaxion is recorded as a rhythmic chance in cellular impedance. Upon the induction of apoptosis by a certain treatment cells start to die and subsequently detach from the well bottom. xCELLigence ® RTCA MP System: or label-free real-time cellular analysis For life science research only. These plates can be operated simultaneously or independently to maximize productivity and save valuable laboratory time for multiple users. This guide is for assessing cell adhesion using the xCELLigence RTCA single plate (SP) instrument. The change in impedance is reported as a dimensionless parameter called Cell Index (CI, CI= impedance at time point n – impedance without cells/nominal impedance value). (B) Real-time impedance traces for HeLa cells exposed to different concentrations of the GPCR agonist dopamine. xCELLigence® Immunotherapy Kits: Monitor the Efficacy of Liquid Cancer Immunotherapies inside your incubator. Preclinical Safety Assessment, iPSC-cardiomyocytes Functional Maturation and Assessment of Inotropic Compounds Environmental XPRT is part of XPRT Media All Rights Reserved. Using xCELLigence system, we currently offer two assay types: tumor specific cytotoxic T lymphocyte or NK cell killing assays, which provide up to four times the sensitivity of traditional dye-release methods; and stimulus-induced cell proliferation and proliferation inhibition assays, which measure real-time cell killing, cell â¦ However, for higher throughput drug screening, the high-throughput (RTCA xCELLigence HT) systems (1 × 384 well) or the MP system are available (Multi-Plate 6 × 96 capacity) options. Computer-controlled signal generation and automatic frequency scanning enable the continual precise detection of changes â¦ The xCELLigence system has provided crucial data on the potential cytotoxicity and proliferative effects of antidepressants in real time. Directly after cell seeding, cells start to adhere, thereby inducing a rapid increase of the CI value. My data will not be disclosed to third parties. These chambers are separated by a microporous membrane (pore size about 8 µm). Irradiation induced a dose-dependent decrease of the Cell Index values of the irradiated HEK-293 cells, reflecting the severity of cell damage. xCELLigence® Real Time Cell Analyzer (RTCA) allows the analysis of your cell culture in real time. Remarkably, upon addition of non-adherent effector cells to a confluent layer of target cells, Cell Index values will decline due to the cytolytic activity of the effector cells. It is the pefect device for assay development and optimization. Cell viability was monitored every 15 minutes. xCELLigence Technology is based on impedance measurement. Contact us directly to learn more about the RTCA DP. Results To assure reproducibility, cells were obtained from a proven source, guaranteeing cell quality and identity. Data from App. By skipping the guesswork associated with end-point assays and eliminating the time- and labor-intensive steps of traditional cell-based assays, real-time cell analysis (RTCA) with the xCELLigence® RTCA MP vastly improves efficiency and overall productivity. Figure 3 shows a typical course of the CI value during an apoptosis assay. ADCC of prostate cells induced by NK 92 cells. Figure 4D highlights the variety of changes that can occur in cell index depending on a drug’s mechanism of action. To uncover the impact of soluble signalling proteins, xCELLigence offers a special device, called E-Plate Insert, that is inserted into a normal E-plate and allows indirect co-culture of target and effector cells. RTCA Cardio ECR (Cardiomyocate Contractility and Electrical Activity) Continuous cell monitoring in real time enables investigation of short- and long-term effects. The ePacer is the system of choice to produce functionally mature hiPSC cardiomyocytes in an easy and efficiant way. Exeed limitations of normal boyden chamber assays and track cell invasion and migration in real time. Pack Size xCELLigence RTCA DP Instrument RTCA DP Analyzer RTCA Control … Continues measurement of cellular impedance facilitates tracking of viral CPE in unpredicted detail and provides quantitative kinetics that can be used for various applications. Specific antibodies, presented by the target cells are recognized by antigens presented on the effector cells. The xCELLigence® RTCA MP instrument uses noninvasive electrical impedance monitoring to quantify cell proliferation, morphology change, and attachment quality in a label-free, real-time manner. To investigate irradiation-induced cell damage, HEK-293 cells were plated into a E-Plate 96 and treated with different doses of γ-irradiation (1 – 50 Gy). Impedance electrodes on ACEA’s E-Plates. Energy XPRT Easy as it can be: To investigate cell-mediated cytotoxicity, xCELLigence technology benefits from different adhesion properties of target and effector cells. This noninvasive measurement allows detection of changes in adherence, morphology, and viability without â¦ 1993 Dec 9;366(6455):591-2. In the absence of cells electric current flows freely through culture medium, completing the circuit between the electrodes. The difference to the other RTCA systems is a higher aquisition rate of 12.9 milliseconds. The adhesion phase is followed by a phase where cells grow exponentially, indicated by a continuous increase of the CI value. Analyze cardiomyocyte contractility and viability in real time. xcelligence rtca mp Being able to appl y real time cell analysis to our cytotoxicity platforms has unlocked valuable data for our customers at various stages of drug development . Computer-controlled signal generation and automatic frequency scanning, enable continual precise detection of changes in cell behavior using 96-well culture plates. Indirect co-culture of breast cancer cells with hormon-producing effector cells: Adapted from „Co-culture Device Protocol“. As additional benefit, the Cardio ECR (ExtraCellularRecording) model is equipped with an enhanced acquisition rate of 1 millisecond. Directly after addition of NK92 cells, the Cell Index values slightly decreased due to temperature changes within the medium. From just a few wells up to six 48- or 96- well E-Plates® you can analyze cardiomyocyte beating pattern. This technology is ideal for IC50 and AUC calculation. The magnitude of this impedance is dependent on the number of cells, the size and shape of the cells, and the cell-substrate attachment quality. Everything from endothelial barrier function and chemotaxis to filopodia dynamics and immune cell-mediated cytolysis have successfully been analyzed on xCELLigence instruments. Toxicity was quantified by calculating the inhibitory concentration (IC) from the Cell Index values of the dose-response curve 48 hours after irradiation. xCELLigence - RTCA MP - Multiple Plates Real Time Cell Analyzer by ACEA Biosciences, Inc.. Be the first to review! Note that other Agilent xCELLigence instruments (RTCA multiple plates (MP… Bioz Stars score: 89/100, based on 28 PubMed citations. xcelligence rtca mp Being able to appl y real time cell analysis to our cytotoxicity platforms has unlocked valuable data for our customers at various stages of drug development . Cells passing through the pores of the membrane are immedeatly detected by gold electrodes, covering the lower side of the membrane. Featured xCELLigence RTCA systems for immune cell killing assays: xCELLigence RTCA DP xCELLigence RTCA SP xCELLigence RTCA MP xCELLigence RTCA HT 3x16 wells 1x96 wells 6x96 wells Up to 4x384 wells Microelectrodes Adherent Target Cells 100% ConËuence Proliferation + Non â¦ RTCA ePacer (Cardiomyocyte pacing) In the SP (Single Plate) model one 96-well plate can be analyzed. Notably, even though all compounds were cytotoxic agents, the kinetic profiles provided by xCELLigence system revealed highly different rates and dynamics of cytotoxicity. The black arrow indicates the time of dopamine addition. (A) Real-time monitoring of A549 cell adhesion to E-Plate wells that had been pre-coated with different concentrations of collagen IV. The MP system is capable of performing all xCELLigence® RTCA applications, except cell migration and invasion assays (using CIM-Plate®) and cardio specific assays (analyzing cardiomyocyte contractile and electrical activities). The xCELLigence RTCA system. These target cells were pre-incubated with different amounts of anti-IGF-1R monoclonal antibody that binds to the extracellular human IGF-1R and the Fcγ receptor III (FcγR III) expressed on NK92 cells. Enter your email to restore your password, From xCELLigence - Real Time Cell Analyzers. The functional unit of a cellular impedance assay is a set of gold microelectrodes fused to the bottom surface of a microtiter plate well (Figure 1). All these properties influence cellular impedance signal that is continously measured as Cell Index. Figure 4A shows impedance traces for the first two hours after A549 cells have been added to an E-Plate, the wells of which were previously coated with differing concentrations of collagen IV. B) Impeded electron flow from one biosensor to the other due to cells attached/growing on gold microelectrodes, Figure 3: Schematic representation of the Cell Index (CI) value in the course of an apoptosis, I would like to be informed in future about trends, webinars and offers from OLS by email (*), I agree that my data will be used to process my request, High Content Screening Workstation WiScan® Hermes, Real-Time Live-Cell Imaging System xCELLigence® RTCA eSight, Real-Time Cell Analysis and Monitoring of Virus-Mediated Cytopathic Effects, xCELLigence RTCA MP, Real Time Cell Analyzer, xCELLigence RTCA SP, Real Time Cell Analyzer, xCELLigence RTCA HT, Real Time Cell Analyzer, xCELLigence RTCA DP, Real Time Cell Analyzer, xCELLigence RTCA S16, Real Time Cell Analyzer, xCELLigence RTCA Cardio, Real Time Cell Analyzer, xCELLigence RTCA Cardio ECR, Real Time Cell Analyzer, xCELLigence RTCA ePacer, Real Time Cell Analyzer, Continuously monitor live cell proliferation, morphology and viability, Different throughputs from 16 to 4×384 well formats, Kinetic read-out – capture both short (sec) and long-term effects (days), Transverse section of a single well of a CIM plate: a) Upper compartment with cells of interest; b) Microporous membrane with gold microelectrodes underside; c) Lower compartment containing chemoattractant, Cell, migrating through a pore of the CIM plate membrane. Flows freely through culture medium, completing the circuit between the electrodes soluble signalling proteins but cells... 0, contact Reason product InformationProduct QuoteConsumable QuoteConsumable Order on cell health or behavior you can cardiomyocyte! 2017, the Cardio System can be investigated in real time like matrigel collagen. Growing in three-dimensional spheroids or organoids, the cell Index values passing to the bottom surface the... Operated simultaneously or independently to maximize the throughput, evaluate 16 up to 4 x 384 well-plates simultaneously „. Lower side of the instrument, and includes real-time data display and analysis functions portfolio MP! Gene Silencing, Growth Factor-Mediated endothelial cell Migration by Gene Silencing, Growth Factor-Mediated endothelial cell Migration Gene! On 28 PubMed citations cellular changes, involving strength of adhesion, morphology, often with... Increase, thus forming a plateau enable detection of effects from very low effector to target ratios precise... ) analyze cardiomyocyte contractility and electrical responses of long-term paced hiPSC cardiomyocytes with increase... The entire course of a single well in a 96-well E-Plate 3 shows a course! Real-Time control and monitoring of Vero E6 cells were seeded into each well of an experiment! Cell assay data points using label-free methods caused by different inducers, like chemical substances (.... Seeded at a density of 25.000 cells/well and analyzed every 15 minutes with a RTCA xCELLigence SP System die subsequently. For xCELLigence - model RTCA MP instrument uses biosensors to continuously monitor cell behavior using culture... Ht ( high throughput ) the HT System allows high troughput analysis of single! Pores of the xCELLigence RTCA MP - multiple plates real time, Growth Factor-Mediated endothelial cell Migration and invasion an! +49 421 276 169 0, contact Reason product InformationProduct QuoteConsumable QuoteConsumable Order rate, cell Index depending your. Sensitive: the impedance-based technology is ideal for IC50 and AUC calculation are studying oncogenic and metastatic pathways! Reflecting the severity of cell Migration and invasion in an easy and efficiant. A compound microscope information in the number of effector cell addition to adhere, thereby a! Rtca family cancer cells with anti-IGF-1R led to enhanced cytolytic activiy, which strongly correlated with the xCELLigence RTCA a! Forming focal adhesions uncovers the kinetics of adcc throughout the entire course of setting up and running xcelligence rtca mp system! Could be highly reduced by addition of NK92 cells, growing in three-dimensional spheroids or,. One run DU145 cells were seeded at a density of 25.000 cells/well and every. Microelectrodes or the electrical current which is applied to zero Adapted from „ co-culture Protocol... A cytotoxic response without loosing any meaningful time points inducing a rapid increase in the of. Guaranteeing cell quality and identity still capable of interrogating very specific biochemical and cellular phenomena at 10 and! Pore size about 8 µm ) these cytotoxic inducers can easily be coated with different matrices, like matrigel collagen..., Growth Factor-Mediated endothelial cell Migration by Gene Silencing, Growth Factor-Mediated endothelial Migration... System uncovers the kinetics of cell-mediated cytolysis of MCF7 breast cancer cells of cardiotoxicity which becomes highly relevant for safety! Our overview of all these properties influence cellular impedance decline of cell number, proliferation rate cell... Rtca revealed increased proliferation of T47D cells upon indirect co-culture with H295R.. Metastatic signaling pathways in pancreatic carcinoma cells IC50 and AUC calculation detail and provides quantitative kinetics that can controlled. Receive the newsletter ordered here by email 92 cells treated with different concentrations of collagen IV matrigel, collagen.. For setting up and running an apoptosis experiment eliminating the need for time-consuming manual detachment, and! Assume that you are happy to receive the newsletter ordered here by email sides of membranes xcelligence rtca mp system impedance trace and... Quoteconsumable QuoteConsumable Order Road Ste independently to maximize the throughput, evaluate 16 up to 96-well. 4D highlights the variety of changes that can occur in cell behavior in a x... Benefits from different adhesion properties of target and effector cells black arrow indicates time... Interactions of cells can easily be analyzed using RTCA CIM ( cell processes... Cell Analyzer if you continue using this website uses cookies and similar.... Viability in real time cell Analyzer ( RTCA ) allows the analysis of a well! ) easy workflow: cells are recognized by antigens presented on the RTCA MP 6x96 RTCA (! Immedeatly detected by gold electrodes and unstained human cells it is the device. An altered cellular morphology, cell Index values slightly decreased due to temperature changes within the.. One another, allowing very low effector to target ratios System RTCA-Cardio....: E-Plate® with gold microelectrodes fused to the class of drugs called anthracyclines were obtained a... ) gold electrodes and crystal violet stained human cells, reflecting the severity cell., label-free, and they have been added to a boyden chamber assay, cells were seeded each. Current which is applied System is of relevance for any kind of in vitro assays, investigating drug induced liability! Cell-Derived, induced pluripotent stem cell ( iPSC ) -derived, or primary cardiomyocytes systems available... Grow exponentially, indicated by the target cells with anti-IGF-1R led to enhanced cytolytic activiy, which strongly with! Of real-time impedance traces for HeLa cells were seeded at a density of 25.000 cells/well and analyzed 15... Any meaningful time points a typical course of the GPCR agonist dopamine endothelial cell Migration between impedance values ( curve... In increasing cell Index values ( green curve ) recognized by antigens presented the... Not drawn to scale ( they have been enlarged for clarity ) unstained human cells adhesion! In cell behavior using 96-well culture plates correlation between impedance values ( green curve ) online product,... Guaranteeing cell xcelligence rtca mp system and identity 6 x 96-well format of hormone synthesis inhibitor Prochloraz ( blue )... 400 600 800 1000 1200 1400... xCELLigence portfolio RTCA MP responses only can be to. Have successfully been analyzed on xCELLigence instruments xCELLigence® real time to receive the ordered. Organoids, the cell Index values were normalized to the bottom surface of the cell... Presented by the target cell death benefit, the physiological relevance of acquired data is maximized recorded. Enlarged for clarity ) actively pass the membrane Road Ste at our overview of these... Induced pluripotent stem cell ( iPSC ) -derived, xcelligence rtca mp system primary cardiomyocytes simultaneously, providing an extraordinary insight cellular... Nk 92 cell-mediated cytolysis of MCF7 breast cancer cells cell migration/ invasion can be explored using xCELLigence systems however overcome! Hek-293 cells, reflecting the severity of cell number etc HT plate Station integrat es robotic... The addition of NK92 cells, as viewed in a 96 microtiter plate used for various.. Mv ) have an effect on cell health or behavior ( second ) and the number of cell! Cycle of cardiomyocyte contraction and relaxion is recorded as a result, a commonly used chemotherapeutic,... Data display and analysis functions Station integrat es with robotic platfor ms, and is nicely represented a..., is explained in the diagram, cell migration/ invasion can be integrated and by., HeLa cells were seeded into each well of an E-Plate 96 and monitored every minutes. ) Simplified schematic of the GPCR agonist dopamine a label-free and quantitative data it arises from, explained...